Journal: International Journal of Molecular Sciences
Article Title: What Drives Symbiotic Calcium Signalling in Legumes? Insights and Challenges of Imaging
doi: 10.3390/ijms20092245
Figure Lengend Snippet: Choices in image processing alter the final ratio image, especially at its edge. The position of a nucleus (yellow) in a M. truncatula root hair is shown in the bright field image of the root hair (grey), merged with the YFP image. Path A shows what happens when images are not processed, while Path B attempts to follow the image processing steps reported in Sieberer et al. . The 2D microscope image of the YFP and CFP intensities at the nucleus reflects the 3D structure of the nucleus, I 1 and I 2 B . The ratios YFP/CFP of images are I 2 A and I 3 B . We used the nuclear localised Ca 2 + sensor YC2.1.
Article Snippet: We imaged the root hairs using a confocal microscope (Zeiss Axio Imager Z2 upright microscope, LSM 780), and as Ca 2 + sensors, we used members of the Yellow cameleon (YC) family of genetically-encoded calcium sensors, specifically YC2.1 (Figures 4 and 5) and YC3.6 (Figures 6 and 8).
Techniques: Microscopy